Avaliação da atividade biológica de preparações homeopáticas de antiadiponectina em camundongos submetidos a dieta hipercalórica / Biological activity of homeopathic preparations of adiponectin in mice subjected to a hypercaloric diet

A adiponectina foi originalmente identificada como uma proteína expressa e produzida por adipócitos do tecido gorduroso abdominal de várias espécies animais, cuja função é manter a homeostase do tecido adiposo em relação às demandas energéticas do corpo. Preparações homeopáticas de substâncias endógenas são capazes de modular diferentes funções celulares. Neste projeto, buscou-se conhecer os possíveis efeitos de uma preparação homeopática de anticorpos antiadiponectina no controle do depósito adiposo subcutâneo abdominal, utilizandose modelo experimental. Quarenta camundongos Swiss machos adultos oram divididos em um grupo experimental e 3 controles. Os animais foram alimentados durante 1 mês e 7 dias com suplemento dietético comercial e tratados simultaneamente, por via oral, com antiadiponectina 6cH. Foi avaliado o ganho de peso corporal, bem como características macro e microscópicas do tecido adiposo subcutâneo e do fígado. Os resultados mostraram discreta redução no ganho de peso e redução estatisticamente significativa no diâmetro dos adipócitos no grupo experimental. Não foram observadas alterações inflamatórias ou degenerativas significativas no fígado. Os mecanismos envolvidos nestas alterações metabólicas precisam ser pesquisados em estudos futuros. (AU)

Adiponectin was originally identified as a protein expressed and produced by the abdominal fat adipocytes of several animal species which function is to maintain the homeostasis of the adipose tissue vis-à-vis the body energy demands. Homeopathic preparations of endogenous substances are able to modulate several cell functions. In the present study we investigated possible effects of a homeopathic preparation of anti-adiponectin antibodies on the control of the abdominal fat by means of an experimental model. Forty Swiss male adult mice were fed a commercial dietary supplement for 1 month and 7 days and treated with anti-adiponectin 6cH per oral route. Parameters assessed were weight gain and macro- and microscopic characteristics of the subcutaneous adipose tissue and liver. The experimental group exhibited slightly less weight gain and statistically significant reduction of the adipocyte diameter. We did not detect significant inflammatory or degenerative changes in the liver. The mechanisms involved in the detected changes need to be investigated in future studies. (AU)

Rats Born to Mothers Treated with Dexamethasone 15 cH Present Changes in Modulation of Inflammatory Process.

As little information about the effect of ultra high dilutions of glucocorticoid in reproduction is available in the literature, pregnant female Wistar rats (N = 12) were blindly subcutaneously treated during all gestational and lactation period with: dexamethasone 4 mg/kg diluted into dexamethasone 15 cH (mixed); or dexamethasone 4 mg/kg diluted in water; or dexamethasone 15 cH, or vehicle. Parental generation had body weight, food and water consumption monitored. The F1 generation was monitored regarding to newborn development. No birth occurred in both groups treated with dexamethasone 4 mg/kg. After 60 days from birth, 12 male F1 rats were randomly selected from each remaining group and inoculated subcutaneously with 1% carrageenan into the footpad, for evaluation of inflammatory performance. Edema and histopathology of the footpad were evaluated, using specific staining methods, immunohistochemistry and digital histomorphometry. Mothers treated with mixed dexamethasone presented reduced water consumption. F1 rats born to dexamethasone 15 cH treated females presented significant increase in mast cell degranulation, decrease in monocyte percentage, increase in CD18+ PMN cells, and early expression of ED2 protein, in relation to control. The results show that the exposure of parental generation to highly diluted dexamethasone interferes in inflammation modulation in the F1 generation.